Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user’s own risk. The ReadyMix contains KAPA HiFi HotStart DNA Polymerase (0.5 U per 25 µL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (2.5 mM at 1X) and stabilizers. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared to wild-type B-family DNA polymerases. KAPA HiFi HotStart DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA, without the need for accessory proteins or DNA binding domains. 05427ES–021 - enter the lot number 05427ES without the filling-code Kapa Biosystems offers a robust portfolio of DNA polymerase reage...Keywords: Amplification, Centrifugation, Cloning, DNA purification, Gas chromatography, Genetic, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Purification, Reductions, Sample preparations, Sequencing, Size-exclusion chromatography, Solvents, We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Nevertheless, always ensure that the KAPA HiFi HotStart ReadyMix is fully thawed and thoroughly mixed before use. Each batch of KAPA Taq EXtra HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). NOTE: Since bisulte treatment is typically performed after library construction, libraries for bisulte conversion must be prepared with adapters containing methylated cytosine residues. The ReadyMix contains KAPA HiFi HotStart DNA Polymerase (0.5 U per 25 µL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl 2 (2.5 mM at 1X) and stabilizers. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 1 PubMed citations. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets. Be the first to write a review! POD 11020REV >> Next-Generation PCR Product KAPA2G Robust KAPA2G Fast KAPA HiFi Sample product codes KAPA2G Robust 89125-054 KAPA2G Robust HotStart 89125-062 KAPA2G Robust HotStart ReadyMix 89125-076 KAPA2G Fast 89125-080 KAPA2G Fast HotStart 89125-088 KAPA2G Fast HotStart ReadyMix 89125-110 KAPA HiFi 89125-032 KAPA HiFi HotStart 89125-036 KAPA HiFi HotStart ReadyMix … The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. P260 - P264 - P270 - P301 + P312 + P330 - P308 + P311 - P501, Kapa Biosystems Taq DNA Polymerase and PCR Kits. Like wild-type Taq, KAPA2G Fast has 5’→3’ polymerase and 5’→3’ exonuclease activities, but no 3’→5’ exonuclease (proofreading) activity. KAPA HiFi exhibits robust performance on targets with high GC-content. ZERO BIAS - scores, article reviews, protocol conditions and more Using the KAPA2G Fast PCR Kit, PCR time ca...Keywords: AGE, Amplification, Buffers, Cloning, Detergents, Electrophoresis, Gel electrophoresis, Genetic, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Purification, Reductions, Sequencing, Size-exclusion chromatography, DNA extraction and amplification are critical steps in your molecular biology workflow and often requires purified, high-quality DNA. Substance name : KAPA HiFi HotStart ReadyMix (2X) (BM0039) Hazardous ingredients Chemical name CAS-No. Kits also include Not for use in diagnostic procedures. • Both … Reproduction of any materials from the site is strictly forbidden without permission. KAPA HiFi HotStart ReadyMix is designed for routine, high-fidelity PCR of a … intrinsichigh processivity enzymeresults signicantimprovement yield,speed sensitivitywhen compared wild-typeB-family DNA polymerases. Nevertheless, always ensure that the KAPA HiFi HotStart ReadyMix (2X) is fully thawed and thoroughly mixed before use. Amplification of bisulfite-converted human genomic DNA targets using Q5U Hot Start High-Fidelity DNA Polymerase (Q5U), Phusion U Hot Start DNA Polymerase (P) and KAPA Hifi HotStart Uracil+ ReadyMix PCR Kit (K). Contact Bioz Stars score: 90/100, based on 1 PubMed citations. KAPA2G Fast HotStart DNA Polymerase with dNTPs - Mg2+ free buffer (250 U) (Kapa Biosystems) View Detail Add to Order. Cycling parameters 4.1 Getting started: Standard 3-step cycling profiles with short extension times are recommended as a starting point for KAPA2G Fast HotStart assays. If your These kits contain all the components required for amplification, including the KAPA HiFi enzyme (either non-HotStart or HotStart), KAPA HiFi buffers, MgCl 2 and dNTPs. -021. KAPA HiFi (High Fidelity) HotStart ReadyMix 100 x 25uL reactions 1 x 1.25/6.25mL Engineered DNA Polymerases Improve NGS and Enable Novel Applications Product Overview Applications which are likely to require higher MgCl 2 concentrations include long PCR (>10 kb) and AT-rich PCR, as well as amplification using primers with a low GC content (<40%). to you upon submission of this form. The LD-PCR mastermix contained 6.25 µl 2x KAPA HiFi HotStart ReadyMix (Roche Diagnostics), 0.125 µl 20:µM PCR_Satija forward primer (Table S3), 0.125 µl 20 µM SmarterR reverse primer8 (Table S3), and 1 µl PCR-grade H2O (Qiagen). The double-stranded cDNA was further amplified in a 50 μl PCR reaction containing 2x KAPA HiFi HotStart ReadyMix (Kapa Biosystems) and 10 μM ImSTRT-TSO-PCR with the following thermocycling parameters: 1 cycle of 3 min at 95°C, 20 cycles of 20 s at 95°C, 15 s 55°C, 30 s at 72°C, and 1 cycle of final elongation of 1 min at 72°C. Return to -20°C for long-term storage. Need larger quantities for your development, manufacturing or research applications? The HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. presented with a COA Request form. to you upon submission of this form. The first PCR mixture comprised 0.4 μL of 10 μM primer mix (5′ WTA and Trbc_ex), 4.6 μL of template, and 5 μL of KAPA Hifi Hotstart ReadyMix (KAPA Biosystems). More important for COO: enter a "0" if only two numbers are Concentration (% w/w) Glycerol 56-81-5 … STBB0728K9 - enter the lot number STBB0728 without the filling-code Note: KAPA Express Extract is not suitable for DNA extraction from plants; for plant PCR applications, the KAPA3G Plant PCR Kit (08 041 091 001) is recommended. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets. KAPA HiFi HotStart Uracil+ ReadyMix contains Mg 2+ at a 1X concentration of 2.5 mM, which is optimal for most applications. Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. KAPA HiFi HotStart is also available in KAPA HiFi HotStart ReadyMix (2X), which contains DNA Polymerase, reaction buffer, dNTPs and MgCl 2 (at a final concentration of 2.5 mM). Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). KK5517. P260 - P264 - P270 - P308 + P311 - P405 - P501, Kapa Biosystems Taq DNA Polymerase and PCR Kits. Each 25 μl reaction contained 12 ng of bisulfite-converted DNA (Qiagen). PEG/NaCl Solution does not freeze at -15°C to -25°C, but should be equilibrated to room temperature and mixed thoroughly before use. in front of the letter e.g. HOTSTART is a registered trademark of Molecular BioProducts, Inc. ReadyMix is a trademark of Sigma-Aldrich Co. LLC. 62K1064 – you will only find the COO if KAPA HiFi HotStart ReadyMix (2X) contains isostabilizers and may not freeze completely, even when stored at -15°C to -25°C. KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. KK5512. you enter 062K1064. .. Cycling conditions were as follows: 95 °C for 3 min, 35 cycles of 98 °C for 20 s, 60 °C for 15 s, and 72 °C for 15 s. KAPA HiFi DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA, without the need for accessory proteins or DNA binding domains. KAPA2G Robust HotStart DNA Polymerase (500 U) (Kapa … Copyright Consent | These characteristics are retained in KAPA HiFi Uracil+, a modied version of KAPA HiFi engineered Reactions may be supplemented with any PCR-grade MgCl 2 solution. Delivered in 2 to 3 days (estimated) $344.96 Add to Order: KK5512. KAPA HiFi HotStart ReadyMix (2X) GHS Classification Specific target organ systemic toxicity - single exposure Category 1 H370: Causes damage to organs. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols. Article Snippet: Genomic DNA sites in KLF4, HBB , and RHOXF2 were amplified by PCR using bisulfite-converted gDNA as a template with Kapa HiFi HotStart Uracil+ ReadyMix (Kapa Biosystems) (for KLF4 and HBB sites) or Qiagen’s PyroMark PCR Kit (for RHOXF2 sites). KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. KAPA2G Robust HotStart ReadyMix enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. Reactions contained 1× KAPA HiFi buffer, 0.3 mM KAPA dNTP mix, 0.3 μM each primer, 1 µl diluted cDNA, 0.5 U KAPA HiFi HotStart DNA polymerase, and PCR-gradewater to 25 µl. number 09019TO without the first two letters. The remaining 23 μl of the samples was then amplified with Kapa Hifi hotstart readymix (we used 7 cycles for all samples in this study). colony PCR) can be used as template.• Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates.• KAPA2G Robust HotStart ReadyMix contains 2 mM MgCl2 at 1X.• KAPA2G Robust HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.• KAPA2G Robust HotStart ReadyMix is ideal for single-protocol PCR, i.e. Site Use Terms following the words 'Lot' or 'Batch'. KAPA HiFi HotStart ReadyMix(2X) contains isostabilizers and may not freeze completely, even when stored at -15°Cto-25°C. Long-term storage at room temperature and 4°C is not recommended. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. KAPA HiFi NGS Library Amplification Kits contain KAPA HiFi HotStart ReadyMix (2X), a ready-to-use cocktail containing all components for PCR, except primers and template. Contact Fast PCR is PCR in which total reaction time is decreased by reducing the duration of one or more of the steps during each cycle of a PCR. Bioz Stars score: 97/100, based on 1 PubMed citations. Nevertheless, always ensure that the KAPA HiFi HotStart ReadyMix (2X) is fully thawed and thoroughly mixed before use. using KAPA Express Extract. Reproduction of any materials from the site is strictly forbidden without permission. ZERO BIAS - scores, article reviews, protocol conditions and more The 2X KAPA Taq EXtra HotStart ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- … If you find a lot number with a filling-code such as If you find a lot number with a filling-code such as • KAPA2G Fast ReadyMix contains 1.5 mM MgCl 2 at 1X.KAPA2G Fast ReadyMix includes two inert tracking dyes to allow direct loading onto agarose gels. If you find a lot number such as TO09019TO - enter the lot Amplicon name and sizes are indicated above the gel. In some cases, a COA may not be available on-line. Reduce cycling times up to 75% :• Extension times as low as 1 sec per kilobase• Broad coverage of both AT- and GC-rich targetsHigh speed, higher performance :• Single copy sensitivity from complex targets• Increased yieldsQuick Notes :• KAPA2G Fast HotStart ReadyMix PCR Kits contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.• Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.• No need for specialized instrumentation or PCR consumables.• Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.• For high reaction efficiency, do not exceed 25 μL reaction volumes.• KAPA2G Fast HotStart ReadyMix contains 1.5 mM MgCl2 at 1X.• KAPA2G Fast HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.Reaction products are 3’-dA-tailed and may be cloned into TA cloning vectors. KAPA2G Fast ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. The PEG/NaCl Solution does not freeze at -15°C to -25°C, but should be equilibrated to room temperature and mixed thoroughly before use. KAPA2G Fast HotStart DNA Polymerase with dNTPs - Mg2+ free buffer (250 U) (Kapa Biosystems) View Detail Add to Order. Citations: Get Quote. Proteins, the functional workhorses of living organisms, are composed of chains of interlinked amino acids that together form peptides and ultimately dictate the three-dimensional structure and funct... What is Fast PCR? Additional MgCl 2 may be added separately, but is unlikely to be required. KAPA HiFi HotStart ReadyMix is designed for routine, high … Questions? amplification of fragments varying from 25–65% GC, up to 1 kb in size, using a single reaction setup and cycling protocol.• Reaction products are 3’-dA-tailed and may be cloned into TA cloning vectors. number 09019TO without the first two letters. KAPA HiFi HotStart ReadyMix contains a final (1X) MgCl 2 concentration of 2.5 mM, which is sufficient for most applications. and Ligation Module kits for end modification, using the 2X KAPA HiFi HotStart ReadyMix for ligation-mediated PCR (LM-PCR),as well as using NimbleGen liquid probe sets for solution- based single or co-capturing target enrichment. In addition, the ability to amplify long targets, as … The name of this product was recently changed. When stored under these conditions KAPA HiFi HotStart ReadyMix PCR Kit. The above denaturation parameters must therefore be strictly adhered to. Explore our integrated Roche Sample Preparation Solutions, encompassing all the steps required to convert a sample to a sequencing-ready library. KAPA HiFi HotStart ReadyMix is designed for routine, high … If you find a lot number such as TO09019TO - enter the lot For cDNA amplification, 7.5 µl LD-PCR mastermix were added to each well. For Research Use Only. Bioz Stars score: 97/100, based on 1 PubMed citations. © 2021  Merck KGaA, Darmstadt, Germany and/or its affiliates. KAPA 2G™ Fast HotStart ReadyMix (2X) 2 4. 05427ES–021 - enter the lot number 05427ES without the filling-code KAPA2G Robust PCR Kits are unique as they contain the KAPA2G Robust DNA Polymerase, a second-generation enzyme that was engineer...Keywords: Amplification, Buffers, Cloning, Detergents, Digestions, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Purification, Sequencing, Size-exclusion chromatography, DNA extraction and amplification are critical steps in your molecular biology workflow and often requires purified, high-quality DNA. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.KAPA2G Fast HotStart® DNA Polymerase is supplied in a convenient 2X ReadyMix format, containing all reaction components except primers and template. you enter 062K1064. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme unt… Kapa Biosystemssupplier Diversity Partner Hifi Hotstart Readymix 500rxn Fisher Scientific, supplied by Fisher Scientific, used in various techniques. Nevertheless, always ensure that the KAPA HiFi HotStart ReadyMix is fully thawed and thoroughly mixed before use. KAPA2G Fast HotStart® ReadyMix™ has been used for:• Routine PCR• Fast PCR• Reverse transcription polymerase chain reaction (RT-PCR)• Genotyping and fluorescent PCR. Kapa Htp Protocol, supplied by Kapa Biosystems, used in various techniques. KAPA HiFi HotStart ReadyMix reduces amplification bias and improves sequencing coverage. KAPA HiFi HotStart ReadyMix yielded significantly more even coverage of the C. difficile genome than NEB Q5. Libraries were prepared for whole-genome shotgun sequencing from 100 ng of Covaris-sheared human genomic DNA using either the KAPA HTP Library Preparation Kit (first) or a competitor kit (second). | Privacy. KAPA HiFi has been engineered to have an increased affinity for DNA resulting in significant improvements to yield, sensitivity, speed, and the ability to amplify difficult templates. Genomic DNA sites in KLF4, HBB , and RHOXF2 were amplified by PCR using bisulfite-converted gDNA as a template with Kapa HiFi HotStart Uracil+ ReadyMix (Kapa Biosystems) (for KLF4 and HBB sites) or Qiagen’s PyroMark PCR Kit (for RHOXF2 sites). The ReadyMix contains KAPA HiFi HotStart DNA Polymerase (0.5 U per 25 µL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (2.5 mM at 1X) and stabilizers. Always ensure that the product has been fully thawed and mixed before use. Since buffer composition affects DNA melting, it is important to denature complex templates and targets with a high GC content sufficiently. KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA po… Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others. 2GFHSRMKB - KAPA2G Fast HotStart ReadyMix, Pricing & availability is not currently available. If you find a lot number with a filling-code such as 4 KAPA HiFi HotStart ReadyMix contains 0.3 mM of each dNTP (1X), and 0.5 U of KAPA HiFi HotStart DNA Polymerase (per 25 µL reaction) in … HotStart ReadyMix is recommended for the rapid and . 62K1064 – you will only find the COO if KK5512. Kapa Hifi Hotstart Readymix, supplied by Kapa Biosystems, used in various techniques. KAPA HiFi DNA Polymerase is designed for low-bias, high-fidelity PCR, and is the reagent of choice for NGS library amplification.1,2,3,4 KAPA HyperPlus Kits include KAPA HiFi HotStart ReadyMix (2X), a ready-to-use PCR mix comprising all the components for … Long-term storage at room temperature and 4°C is not recommended. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared to wild-type B-family DNA polymerases. Long-term storage at room temperature or 4 °C is not recommended. -021. KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). 0110 Lit. Each batch of KAPA2G Robust HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. KAPA2G Robust HotStart ReadyMix PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels. More important for COO: enter a "0" if only two numbers are 1 The KAPA HiFi HotStart ReadyMix Buffer has a higher salt concentration than conventional PCR buffers. • Reaction products are 3’-dA-tailed and may be cloned into TA cloning vectors. KAPA HiFi HotStart Uracil+ ReadyMix (2X) is a ready-touse cocktail containing all components required for PCR, except primers and template. The old name was: KAPA High Fidelity HotStart Uracil with ReadyMix KAPA2G Robust HotStart® ReadyMix™ enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk. All Rights Reserved. Not for use in diagnostic procedures. amplifylong targets, AT-richtargets, … Return to -20°C for long-term storage. The ReadyMix contains KAPA HiFi HotStart DNA Polymerase (0.5 U per 25 µL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl 2 K9. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. KAPA HiFi HotStart Uracil+ ReadyMix (1 x 1.25 mL) (Kapa Biosystems) View Detail Add to Order. KAPA HiFi HotStart Uracil+ ReadyMix (1 x 1.25 mL) (Kapa Biosystems) View Detail Add to Order. Customer Service. Lot and Batch Numbers can be found on a product's label KAPA HiFi HotStart ReadyMix reduces amplification bias and improves sequencing coverage. The ReadyMix contains 0.2 mM of each dNTP (dATP, dCTP, dGTP, dTTP), and does not contain dUTP. MSDS KAPA HiFi HotStart ReadyMix PCR Kits v1.09 Page 1 of 5 1 – 5% MATERIAL SAFETY DATA SHEET KAPA HiFi HotStart ReadyMix PCR Kits Prepared in accordance with EU directive 91/155/EEC. KAPA HiFi achieved a 100% success rate with higher yields up to 84% GC content. Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. KAPA HiFi PCR Kits and KAPA HiFi HotStart PCR Kits are provided in a component format to allow flexibility and optimization for a range of PCR applications in both PCR and NGS workflows. Site Use Terms Bioz Stars score: 86/100, based on 1 PubMed citations. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). STBB0728K9 - enter the lot number STBB0728 without the filling-code Home > Search Results > Kapa Biosystems > kapa htp protocol. The ReadyMix contains the novel KAPA2G Robust HotStart polymerase, engineered for improved processivity and tolerance to common PCR inhibitors through a process of directed evolution. KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading fidelity and performance when compared with other proofreading polymerases and polymerase blends. Lot and Batch Numbers can be found on a product's label 1 $283.24 Add to Order: KK5517 . K9. search was unable to find the COA you are looking for, you will be KAPA HiFi HotStart HotStart ReadyMix (2X) may be stored at 4 °C for regular, short-term use (up to 1 month). All Rights Reserved. No. Provided that it has been handled carefully and not contaminated, the ReadyMix is not expected to be compromised if left (unintentionally) at room temperature for short periods of time (up to 3 days). Kits include KAPA HiFi or KAPA HiFi HotStart DNA Polymerase (1 U/µL), Fidelity Buffer (5X), GC Buffer (5X), high-quality dNTPs (10 mM each) and MgC l2 (25 mM). Both buffers contain MgCl 2 at a final concentration of 2 mM. The PEG/NaCl Solution does not freeze at -15°C to -25°C, but should be equilibrated to room temperature and mixed Reagents may be stored at 4°C for short-term use (up to 1 month). search was unable to find the COA you are looking for, you will be 2+.. KAPA3G HotStart DNA Polymerase is an antibody-mediated HotStart 3rd generation mutant of Taq specifically designed for fast and inhibitor-resistant PCR and formulated without glycerol for preparation of dried amplification mixes. A panel of human amplicons with GC contents ranging from 66 – 84% was used to compare the performance of KAPA HiFi with that of an engineered polymerase with fusion technology and wild-type Pfu. Always ensure that the product has been fully thawed and mixed before use. Efficient amplification of GC- and AT-rich targets• Robust performance across a wide range of GC- and AT-rich templates• Increased PCR success ratesImproved tolerance to common PCR inhibitors• Efficient amplification from crude samples• Higher yield and sensitivity per unit of enzymeUnrivalled performance in colony PCR• Higher yields and improved consistency of PCR direct from E. coli and yeast cellsQuick Notes:• KAPA2G Robust HotStart ReadyMix PCR Kits contain KAPA2G Robust DNA Polymerase, engineered for high processivity and inhibitor tolerance.• Both purified genomic DNA and crude samples (e.g. KAPA HiFi HotStart Uracil+ ReadyMix is typically used to amplify (enrich) bisulteconverted, adapterligated library fragments prior to quantication and sequencing. 2X KAPA HiFi HotStart Uracil+ ReadyMix KK2802 contains 2.5 mM MgCl2 at 1X (6.25 ml) Quick Notes • KAPA HiFi provides high delity NGS library amplication with the highest yield, lowest bias, and most uniform sequence coverage available. 2GRHSRMKB - KAPA2G Robust HotStart ReadyMix, Pricing & availability is not currently available. KAPA Express Extract. Reagents may be stored at 4°C for short-term use (up to 1 month). KAPA HiFi HotStart ReadyMix (2X) may not freeze completely, even when stored at -15°C to -25°C. Kapa/Roche Kit Codes and Components KK5706 07961391001 (2.5 mL) KAPA2G Robust HotStart ReadyMix with dye (2X) (contains 2 mM MgCl 2 at 1X) Quick Notes • KAPA2G Robust HotStart ReadyMix with dye PCR Kits contain an antibody-mediated hot-start formulation of KAPA2G Robust DNA Polymerase, engineered for high processivity and inhibitor tolerance. Customer Service. Roche Diagnostics HotStart ReadyMix with dye | 100x25uL Reactions Manufacturer: Roche Diagnostics 07961260001 KAPA2G Fast PCR Kits contain a second-generation (2G) DNA polymerase enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. Packaging • KAPA2G Fast Standard or HotStart DNA Polymerase (5 U/μL) • 5X KAPA2G Buffer A with MgCl 2 Kits include KAPA HiFi or KAPA HiFi HotStart DNA Polymerase (1 U/µL), Fidelity Buffer (5X), GC Buffer (5X), high-quality dNTPs (10 mM each) and MgC l2 (25 mM). ZERO BIAS - scores, article reviews, protocol conditions and more. In many cases a COA can be faxed Roche Diagnostics HotStart ReadyMix with dye | 100x25uL Reactions Manufacturer: Roche Diagnostics 07961260001 KAPA2G Fast PCR Kits contain a second-generation (2G) DNA polymerase enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase.
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